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ZirChrom
ZirChrom HPLC Column ZirChrom-PBD, 5 μm, 4.6 x 250 mm
ZirChrom-PBD is produced by coating ultra-stable zirconia particles with an equally stable extremely thin layer of crosslinked polybutadiene. The chemical selectivity of ZirChrom-PBD columns is similar to that of a traditional C8 or C18 silica based column for non-ionic analytes. In the case of ionizable analytes there are secondary interactions which can be used to fine tune the chromatographic selectivity (band spacing). The surface chemistry of zirconia is very rich and may be used to change chemical selectivity of the column simply through the addition of mobile phase additives. Consider using ZirChrom-PBD with a phosphate buffer if either the tailing of amines or their selectivities are problematic on C18 silica, and explore the full pH range (pH 1-14) to optimize your separation. For example, a 20mM phosphate buffer will produce good peak shapes for many ionizable compounds. Great Peak Shapes for Basic Compounds Easy Method Transfer from ODS Silica pH StNäytä lisää
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