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Tosoh Bioscience
Tosoh Bioscience HPLC Column, Glas, TSKgel Boronate-5PW, 1000 Å Polymer, 10 μm, 5 x 50 mm
Coupling of m-aminoPhenyl boronate to the TSKgel 5PW-type polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate-containing compounds, and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW takes place in basic buffers such as HEPES and morpholine, while desorption takes place in carbohydrate or amine-containing mobile phases like sorbitol or Tris.Show more
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